Fractionation of Skimmilk Proteins by Sephadex Gel Filtration
نویسندگان
چکیده
منابع مشابه
Purification of human plasminogen and plasmin by gel filtration on Sephadex and chromatography on diethylaminoethyl-Sephadex.
Highly purified human plasminogen has been prepared from a variety of plasma fractions and by different purification methods. The two major fractions used have been serum or plasma euglobulin and Cohn plasma Fraction III. The purification methods (1-14) have all yielded products of high purity. Plasminogen useful for further purification and isolation studies can be prepared by the Kline method...
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1. Culture filtrates from Myrothecium verrucaria have been fractionated by gel filtration on Sephadex G-75 to give three major cellulolytic components with molecular weights of about 55000, 30000 and 5300. 2. The middle component has the bulk (90%) of the total carboxymethylcellulase activity and is little affected by exposure to cotton. The other two, which are mainly responsible for the activ...
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We have developed a facile means for the refolding of milligram quantities of purified proteins that employs gel filtration chromatography. We demonstrate by electrophoretic mobility shift and NMR spectroscopy that human ETS-1 protein, bovine ribonuclease A and E. coli integration host factor can be refolded into the native conformation using this technique. We have extended this strategy to th...
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1. Human growth hormone was prepared from acetone-dried residues after extraction of gonadotrophins from pituitary glands. 2. Crude growth hormone was purified by gel filtration on Sephadex, resulting in a product that is soluble in water or 0.5% sodium chloride. It is painless on injection and shows a twofold increase in biological potency. Aggregation of growth hormone on Sephadex columns can...
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A simple, 30-mm method of estimating the binding of bilirubin to serum protein was compared with a quantitative method. Good correlation was found between the two methods, both based on Sephadex gel filtration. The results indicate that the presence of loosely bound bilirubin can be determined with this simple, qualitative, semimicro-scale test. This determination is important for the clinical ...
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ژورنال
عنوان ژورنال: Journal of Dairy Science
سال: 1964
ISSN: 0022-0302
DOI: 10.3168/jds.s0022-0302(64)88732-4